By Ronald B. Corley
Millions of tools were built within the a variety of biomedical disciplines, and people coated during this e-book characterize the elemental, crucial and most generally used equipment in numerous diversified disciplines.
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Extra resources for A Guide to Methods in the Biomedical Sciences
Consequently, a “divide and conquer” approach is often used, in which protein are expressed as individual domains for structural analysis. A protein domain is typically defined as an independent folding unit within an overall protein structure. Thus, proteins can be broken down into their component parts. Individual domains of interest, such as regions that contain catalytic sites of enzymes or protein interaction domains, are crystallized and the structure of these domains independently solved.
Recombinant DNA is usually incorporated into vectors that can be introduced into bacteria, so that the bacteria now contains the gene(s) of interest, genes that they would not normally express. The advent of recombinant DNA technology is one of the most significant developments of century biomedical science. The ability to clone genes led to the establishment of the Biotechnology Industry, which uses cloned genes for enzyme and hormone replacement therapies. For example, before the advent of the biotechnology industry, insulin was purified from natural sources, limiting its availability.
He was to receive his second in 1980 for developing what became the most widely used method for sequencing DNA. The ability to indirectly deduce the sequence of a protein has come a long ways since the 1950s and 1960s, and these original methods are only rarely used today. The most common ways to sequence proteins now is to clone its corresponding cDNA or genomic sequence and deduce the amino acid sequence from the nucleotide sequence. Using this method, the genomes of entire species, including man, have been obtained in a relatively short period of time (see Chapter 2).